平车前提取物对慢性非细菌性前列腺炎大鼠炎症因子的影响

摘要

目的：研究平车前提取物对慢性非细菌性前列腺炎大鼠的治疗作用及其机制。方法60只大鼠按随机数字表法分为6组：正常对照组、模型对照组、舍尼通组和平车前提取物大、中和小剂量组,每组10只。除正常对照组外,其他组用角叉菜胶制备慢性非细菌性前列腺炎大鼠模型。舍尼通组灌胃舍尼通溶液100 mg . kg－1,平车前提取物小、中和大剂量组分别灌胃平车前提取物150,300和600 mg.kg－1,正常对照组和模型对照组给予等容量纯化水。每天给药1次,连续3周。观察大鼠前列腺指数( PI)、前列腺特异性抗原( PSA)和前列腺组织中肿瘤坏死因子-α( TNF-α)、白细胞介素-1β(IL-1β)、环氧化酶-2(COX-2)、前列腺素E2(PGE2)、转化生长因子-β1(TGF-β1)和结缔组织生长因子(CTGF)含量的影响,以及前列腺组织病理形态的变化。结果正常对照组 PI 和 PSA 分别为(0.8±0.3)mg.g－1,(106.5±10.6)pg.mL－1,模型对照组PI和PSA分别为(2.2±0.2)mg.g－1,(319.4±23.4)pg.mL－1,舍尼通组PI和PSA分别为(1.6±0.3)mg.g－1,(179.5±13.7)pg.mL－1,平车前提取物小、中和大剂量组PI分别为(1.8±0.4),(1.3±0.3),(0.8±0.3)mg.g－1,PSA分别为(263.4±28.6),(178.5±21.5),(143.5±12.9)pg.mL－1。与模型对照组比较,中和大剂量平车前提取物显著降低慢性非细菌性前列腺炎模型大鼠PI和PSA( P<0.01或P<0.05),显著降低模型大鼠前列腺组织中TNF-α、IL-1β、COX-2、PEG2、TGF-β1和CTGF的水平( P<0.01或P<0.05),同时显著减轻炎症细胞浸润和间质纤维化的程度。结论平车前提取物对慢性非细菌性前列腺炎大鼠有显著的治疗作用。%Objective To study the effect and mechanism of Plantago depressa Willd. extract on rats with chronic nonbacterial prostatitis. Methods Sixty rats were divided into six groups randomly: normal control group, model control group, cernilton group, high-, medium- and low- dose Plantago depressa Willd. extract group, ten rats in each group. Chronic nonbacterial prostatitis rat model was established in the animals except of normal control group. Rats in the cernilton group were administrated with cernilton solution( 100 mg . kg-1 ) . High, middle and low dose Plantago depressa Willd. extract groups were given 150, 300 and 600 mg . kg-1 of Plantago depressa Willd. extract, respectively. The rats in the normal control and model control group were treated with the same volume of purified water.The administration was performed once every day, and lasted for three weeks.Effects of Plantago depressa Willd.extract on prostate index (PI), prostate specific antigen (PSA), tumor necrosis factorα ( TNF-α) , interleukin-1β ( IL-1β) , cycloxygenase 2 ( COX-2) , prostaglandin E2( PEG2 ) , transforming growth factor-β1( TGF-β1 ) , and connective tissue growth factor ( CTGF ) levels and pathological changes of prostate tissue in rats were observed. Results PI and PSA levels of the normal control group were(0.8±0.3)mg.g-1 and(106.5±10.6)pg.mL-1, respectively;those of the model control group were(2.2±0.2)mg.g-1 and(319.4±23.4)pg.mL-1 respectively;Those of the cernilton group were(1.6±0.3)mg.g-1 and(179.5±13.7)pg.mL-1 respectively;Those of the low-, medium- and high- dose Plantago depressa Willd.extract groups were(1.8±0.4),(1.3±0.3),(0.8±0.3)mg.g-1 and(263.4±28.6),(178.5±21.5), (143.5±12.9)pg.mL-1, respectively.Compared with the model control group, PI and PSA were significantly decreased(P<0.01) , TNF-α, IL-1β, COX-2, PEG2 , TGF-β1 and CTGF levels in the prostate tissues were decreased ( P<0. 01 ) , and inflammatory cell infiltration and fibrosis of prostate tissue was significantly alleviated in the model control group. Conclusion This study confirms Plantago depressa Willd.extract exerts effective therapeutical effect on chronic nonbacterial prostatitis in rats.