双波长超高效液相色谱法测定复方黄根颗粒中没食子酸和齐墩果酸含量∗

摘要

目的：建立超高效液相色谱(UPLC)法同时测定复方黄根颗粒中齐墩果酸和没食子酸的含量。方法采用Agilent Eclipse Plus C18色谱柱，以甲醇-0.1％磷酸为流动相，梯度洗脱，流速：0.2 mL•min－1，波长切换，0~7 min波长270 nm测定齐墩果酸，~14 min波长215 nm测定没食子酸。结果没食子酸和齐墩果酸进样量分别在0.0441~0.3971μg( r＝0.9996，n＝5)，0.0621~0.5587μg(r＝0.9998，n＝5)范围内与峰面积积分值呈良好线性关系，平均回收率分别为100.20％和99.96％；RSD分别为0.45％和0.64％。结论该方法操作简便、重复性好，可以更有效地控制制剂的质量。%Objective To establish an UPLC method for content determination of gallic acid and oleandic acid in compound Huanggen granules. Methods Agilent Eclipse Plus C18 column was used with the mobile phase of methanol-0.1%phosphoric acid by gradient elution. Flow rate was 0.2 mL.min-1 . Detection wavelength was set at 270 nm in the first 7 min for gallic acid and then changed to 215 nm for oleandic acid between 7 and 14 min. Results The linear concentration ranges of gallic acid and oleandic acid were 0.044 1-0.397 1 μg ( r=0.999 6,n=5) and 0.062 1-0.558 7μg ( r=0.999 8,n=5) . Their average recovery was 100. 20% ( RSD=0. 45%) and 99. 96%( RSD=0. 64%) , respectively. Conclusion The extablished method is simple,reproducible and specific, and can be use to the quality control of compound Huanggen granules.