绣球菌原生质体制备及再生条件的初步研究

摘要

To further ameliorate the cultivation characteristics and improve the yield of Sparassis crispa,the condition for preparation and regeneration of protoplasts from Sparassis crispa were preliminary studied.Factors including isotonic solutions,enzyme temperature,pH,enzymolysis time were optimized based on the regeneration amount.Results showed that 2％ lysozyme was used to dissolve the mycelium cell wall of mycelium of S.crispa,and the optimal condition for preparing protoplasts were 0.6 M sucrose as isotonic solution,30 ℃,pH 6.0,3 h.Under this condition,the regeneration amount was 11 850 per mL.%为进一步改善绣球菌栽培特性、提高栽培产量,对绣球菌原生质体制备与再生条件进行了初步探索.以绣球菌原生质体再生量为指标,对等渗剂、酶反应温度、pH和反应时间等影响因素进行了优化.结果表明,采用2％的溶壁酶溶解绣球茵菌丝细胞壁,制备原生质体的最佳条件是,以0.6 mol/L蔗糖为等渗剂,在30℃、pH值6.0环境下反应3h.在此条件下,原生质体的再生量为11 850个/mL.