连翘不同部位连翘苷含量测定及其抗氧化活性的测定

摘要

Inorder to determinethe content of phillyrin and the antioxidant activities of different parts of Forsythia suspensa,ultrasonic extraction was applied to extrat phillyrin,the antioxidant activities were assayed through scavenging effects to DPPH radical,and the content of forsythin of Forsythia suspensa was determined by HPLC.The results showed that the best extraction condition was the methyl alcohol content 75%,withdraws 30 minutes,the material ratio was 8∶1,the extraction number of times was twice.The content of phillyrin in forsythia leaf was higher than forsythia flower and fruit obviously.Both different parts of Forsythia suspensa had antioxidant activity.So the content of phillyrin in forsythia leaf was higher and different parts of Forsythia suspensa had antioxidant activity.%为综合利用连翘,现采用超声波辅助法提取连翘苷,HPLC法测定连翘苷,DPPH自由基清除法评价抗氧化活性,对测定连翘不同部位提取液连翘苷含量及其抗氧化活性进行了研究。结果表明：超声波辅助提取法的最佳提取条件为甲醇含量75%,提取30min,物料比为8∶1,提取次数为2次;连翘叶中连翘苷含量显著高于连翘花和果实;连翘花、果实、叶提取液抗氧化活性较强。表明连翘苷在连翘叶中含量较高,连翘不同部位提取液都具有较强的抗氧化活性。