工布乌头中三种生物碱HPLC法测定优化

摘要

In order to optimize the extracting method of alkaloids in Aconitum kongboense L .,the content of mesaconitine ,talatisamine and aconitine were determined by HPLC .The results showed that 10% ammonia 1 mL ,ether 20 mL ,soak for 24 h ,30 min after ultrasonic treatment could be used to the quantitative analysis of the alkaloids of A conitum kongboense L ..%为优化工布乌头中生物碱的测定方法，采用 H PLC法测定工布乌头中新乌头碱、塔拉萨敏和乌头碱的含量。结果表明：采用10％氨水1 mL、乙醚20 mL ，浸泡24 h后超声处理30 min可有效地对工布乌头中生物碱进行定量分析。