何首乌饮对衰老大鼠脑组织细胞Rb/p53信号转导通路影响

摘要

Objective: To investigate the mechanism of Heshouwuyin in anti-aging in brain of the aging model rats by detecting expression of Rb, p16, p53, p19 and p21 in aging rats brain. Method: Totally 84 of 8 month SD rats, weight 180-220g, were selected and randomly divided into normal group, model group,Heshouwuyin high ,middle, low group, Heshouwuwan group and spontaneous recovery group with 12 rats in each group. The sub · acute aging rats were made by injecting D · gal into abdominal cavity continually. SP immunohistochemistry, Western blotting and RT-PCR were used to observe the differential expression of phosphorylated Rb protein, p16, p53, p19 and p21 in brain tissue. Result: The expression of brain tissue Rb, p53,p16, p19 and p21 in model group rat obviously increased, the expression of pRb obviously decreased ( P＜0.05 );Heshouwuyin and Heshouwuwan group raised the expression of pRb, decreased the expression of Rb,p53 ,p16,p19 ,p21 ( P＜0.05 ) ,and the regulating effect of Heshouwuyin high group was more effective than rest groups (P＜0.05). Conclusion: Heshouwuyin regulates the expression of Rb/p53 signal transduction pathway related gene and protein in brains tissue to defer aging.%目的:检测Rb、p16、p53、p19、p21在衰老大鼠脑组织中的表达,探讨何首乌饮抗大鼠脑组织衰老的机制.方法:选用8周龄SD大鼠84只,体重180-220g,随机分为正常组、模型组、何首乌饮高、中、低剂量组、何首乌丸组、自然恢复组,每组12只,采用D-半乳糖连续腹腔注射建立亚急性衰老大鼠模型.免疫组织化学、Western blotting和RT-PCR法检测pRb、p16、p53、p19、p21在大鼠脑组织的表达差异.结果:模型组大鼠脑组织Rb、p53、p16、p19、p2l表达明显增加,pRb表达明显减少(P<0.05);何首乌饮、何首乌丸组能显著提高pRb的表达,降低Rb、p53、p16、p19、p2l表达(P<0.05),何首乌饮高剂量组调节作用大于其他各组(P<0.05).结论:何首乌饮调节脑组织Rb/p53信号转导通路相关基因和蛋白表达起到延缓衰老的作用.