结肠癌患者 HIF-1αVEGF 和 MMP-9表达及临床意义

摘要

Objective: To explore the expression and clinical significance of hypoxia-inducible factor 1α(HIF-1α), vascular endothelial growth factor (VEGF)and matrix metalloproteinase-9(MMP-9) in co-lonic carcinoma.Method:Immunohistochemistry was used to measure HIF-1α, VEGF and MMP-9 in 56 samples of colonic carcinoma and 40 samples of adjacent tissues.The positive rates and the relationship a-mong HIF-1α, VEGF and MMP-9 were studied .Result:The positive rates of HIF-1α, VEGF and MMP-9 in colonic carcinoma were much higher than those in adjacent tissues ( P<0 .05 ); HIF-1αand MMP-9 closely related with lymphatic metastasis, differentiation degree and Duke stage(P<0.05);VEGF was asso-ciated with lymphatic metastasis and Duke stage (P<0.05);The expression of HIF-1α, VEGF and MMP-9 were independent with age, sexes and tumor size (P>0.05).Conclusion: The expression of HIF-1α, VEGF and MMP-9 are closely related with the occurrence and progress of colonic carcinoma .They all par-ticipate in the metastasis of lymphatic channel .%目的：探讨缺氧诱导因子-1α（ HIF-1α）、血管内皮生长因子（ VEGF ）及基质蛋白酶-9（ MMP-9）在结肠癌患者的表达及临床意义。方法：采用免疫组织化学方法检测56例结肠癌组织及40例癌旁组织HIF-1α、VEGF和MMP-9，比较两组阳性率的差别及HIF-1α、VEGF和MMP-9与结肠癌临床病理特征之间有关系。结果：结肠癌组织HIF-1α、VEGF和MMP-9表达阳性率显著高于癌旁组织（P＜0．05）；HIF-1α和MMP-9与淋巴转移、组织学分化及Duke分期显著相关（P＜0．05）；VEGF与淋巴转移、Duke分期相关（P＜0．05）；HIF-1α、VEGF和MMP-9与年龄、性别及肿瘤大小无关（P＞0．05）。结论：HIF-1α、VEGF和MMP-9与结肠癌的发生、发展关系密切，三者参与了结肠癌的淋巴道转移。