Objective To investigate the onset of rots epilepsy after pilocarpine injection and mossy fiber sprouting in hippocampus zone. Methods 60 rats were randomly divided into two groups: epilepsy group and control group, 30 rats in each group. The neuronal damage, mossy fiber sprouting were detected by Nissl and neo-Timm staining. The ECG was performed in the day of experiment for all the rats. Results After the rats were injected with pilocarpine,93% of them developed epilepsy,and most of them showed spontaneous onset during the chronic period. Timm stainning results showed that there was a significant difference in the neuron deletion in epilepsy group between CA3 region and dentate hilus region, and between CA3 region and CA1 region ( P <0.05 ) ,and there was a significant difference in silver staining granules scoring of mossy fiber sprouting between epilepsy group and control group ( P < 0.05 ). Conclusion The epilepsy models can be induced by pilocarpine injection in rots,with basic pathological characteristics of human epilepsy, which is a useful and simple way for studying epilepsy.%目的 观察腹腔注射匹鲁卡品后大鼠痫性发作及海马苔藓出芽.方法 Wistra大鼠60只随机分为模型组和对照组,每组30只,均进行Nissl染色及Timm银染.并于实验当天行脑电图检查.结果 腹腔注射匹鲁卡品后大鼠可见急性癫痫发作,观察慢性期自发性发作.Nissl染色示模型组各区评分两两比较,结果 示CA3、门区与CA1区神经元缺失差异有统计学意义(P<0.05).Timm银染示2组苔藓纤维出芽Timm银染颗粒评分比较,差异有统计学意义(P<0.05).结论 腹腔注射匹鲁卡品简单复制了人类癫痫的基本病理特征,是研究癫痫的一种简便方法.
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