非小细胞肺癌组织中 PTEN、NF-κB 的表达及临床意义

摘要

Objective To detect the expressions of phosphatase and tensin homology deleted on chromosome ten (PTEN) and nuclear factor-κB (NF-κB) in non-small-cell lung carcinoma (NSCLC) tissues,and to explore their correlation with clinical pathological characteristics as well as patient ’ s prognosis .Methods The immunochemistry method was used to detect the expressions of PTEN and NF-κB in NSCLC tissues and adjacent nontumorous tissues.Results The positive rates of PTEN expression in lung cancer tissues were 24.42%(21/86),which were significantly lower than 72.09% (62/86) in adjacent nontumorous tissues ( P <0.05), however,the positive rates of NF-κB expression in lung cancer tissues were 63.95%( 55/83 ), which were significantly higher than 25.58%( 22/86) in paracancerous tissues ( P <0.05).The positive rates of PTEN and NF-κB expressions were related to clinical pathology staging ,differentiation degree of tissue and survival periods after operation,however,which were not correlated with gender ,age,smoking,tumor size and histological grading ( P >0.05),however,the expression of PTEN was negatively correlated to that of NF-κB ( rs =-11.30, P <0.05).Conclusion The inactivation of PTEN gene and activation of NF-κB gene may promote the pathogenesis , development,invasion and metastasis of lung cancer , which can be used as the indexes to evaluate tumor ’ s malignant degree and prognosis .%目的检测非小细胞肺癌（ NSCLC）组织中张力蛋白同源的磷酸酶基因（ PTEN）和核因子κB （ NF-κB）的表达及其与临床病理特征及预后的关系。方法运用免疫组织化学法检测PTEN、NF-κB 在肺癌组织以及癌旁正常肺组织中的表达情况。结果 PTEN蛋白在肺癌组织中的阳性表达率明显低于癌旁正常肺组织（ P ＜0剟．05）；而NF-κB蛋白在肺癌中的阳性表达率，明显高于癌旁正常肺组织（ P ＜0．05）。PTEN、NF-κB的阳性表达率与临床病理分期、组织分化程度及术后生存期有关（ P ＜0．05），而与性别、年龄、吸烟史、肿瘤大小及病理类型无关（ P ＞0．05）；临床病理分期低、组织分化好的患者生存时间较长，差异有统计学意义（ P ＜0．05）。此外，肺癌中PTEN与NF-κB呈负相关（ rs＝-11．30， P ＜0．05）。结论PTEN基因的失活、NF-κB基因的活化可能促进了肺癌的发生、发展和侵袭转移的进程，可作为临床评价肿瘤恶性程度及预后的指标。