目的：观察表没食子儿茶素没食子酸酯（EGCG）体外增强5-氟尿嘧啶（5-Fu）对食管癌CaEs-17细胞化疗敏感性的作用，并探讨可能的作用机制。方法实验分为空白对照组、5-Fu 组（培养液中5-Fu 终浓度为6μmol/L），EGCG组（培养液中EGCG终浓度为20 mg/L）、联合组（培养液中EGCG终浓度为20 mg/L，5-Fu浓度为6μmol/L），4组作用24 h，CCK-8法和细胞克隆形成法检测细胞增殖抑制率和存活率，金氏公式评价二药的协同效果；流式细胞仪检测细胞凋亡率及周期分布；Transwell法检测细胞侵袭力；免疫印迹法检测突变型P53（mtP53）蛋白表达水平。结果 EGCG、5-Fu均可抑制CaEs-17细胞增殖、诱导细胞凋亡、降低细胞存活率和侵袭力；EGCG、5-Fu均可阻滞细胞于G0/G1期，并能下调mtP53蛋白表达水平（ P ＜0．05），二药联用上述作用效果更为显著（ P ＜0．01）。结论 EGCG能够增强5-Fu对食管癌CaEs-17细胞的化疗敏感性，这种作用部分是通过下调mtP53蛋白实现的。%Objective To observe the effect of epigallocatechin-3-gallate ( EGCG ) in enhancing chemotherapy sensitivity of 5-fluorouracil ( 5-Fu ) on human esophageal cancer line-CaEs-17 in vitro, and to explore possible action mechanism.Methods The experiment consisted of four groups:blank control group,5-Fu group ( final concentration of 5-Fu:6μmol/L in culture medium),EGCG group (final concentration of EGCG:20mg/L),combination group (final concentration of EGCG:20mg/L and 5-Fu:6μmol/L),affected for 24 hours in four groups.The inhibitory rate on cell proliferation and survival rate were detected by CCK-8 method and cell clone formation test,respevtively;the synergism effect of two drugs was evaluated according to Jin’ s formula;the cell apoptosis rate and cell cycle distribution were detected by flow cytometry;cell invasiveness was determined by Transwell method;the expression levels of mutant P53 (mtP53) protein were detected by Western Blot. Results Both EGCG and 5-Fu could inhibit the proliferation of CaEs-17 cells,induce cell apoptosis ,decrease cell survival rate and cell invasiveness, moreover, both EGCG and 5-Fu could block cell at G0/G1 stage and cloud down-regulate the expression levels of mtP53 protein ( P <0.05),the effects of combined application of two drugs were more obvious ( P <0.01).Conclusion EGCG can enhance chemotherapy sensitivity of 5-Fu on human esophageal cancer line-CaEs-17 in vitro, which is performed partly through down-regulating the expression levels of mutation P53 protein.
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