热喘平口服液对支气管哮喘豚鼠一氧化氮、肿瘤坏死因子-α、超氧化物歧化酶及丙二醛的影响

摘要

目的 探讨热喘平口服液对实验性支气管哮喘豚鼠的平喘作用机制.方法 将72只雄性豚鼠,随机分为正常对照组、模型组、热喘平口服液低剂量组、热喘平口服液高剂量组、地塞米松组及中西药结合组,每组12只.除正常对照组外其余5组均予10％卵白蛋白1 mL腹腔注射造模.热喘平口服液低剂量组予热喘平口服液[按10 g／(kg·d)相当于生药3 g／d,制成6 mL口服液]灌胃给药.热喘平口服液高剂量组予热喘平口服液[按20 g／(kg·d)相当于生药6 g／d,制成6 mL口服液]灌胃给药.地塞米松组予地塞米松磷酸钠注射液[按3．5 mg／(kg·d)相当于地塞米松1 mg／d,制成6mL混悬液]灌胃给药.中西药结合组予热喘平口服液[按20 g／(kg·d)相当于生药6g/d,制成3 mL口服液];地塞米松磷酸钠注射液[按3．5 mg／(kg·d)相当于1 m/d,制成3 mL混悬液]灌胃给药.正常对照组及模型组均予0．9％氯化钠注射液灌胃给药.各组均灌胃7 d后检测支气管哮喘豚鼠支气管肺泡灌洗液中一氧化氮(N0)、肿瘤坏死因子-α(TNF-α)及血清中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量.结果 模型组,地塞米松组,中西药结合组及热喘平口服液高、低剂量组NO、TNF-α均高于正常对照组(P<0．05);地塞米松组,热喘平口服液高、低剂量组及中西药结合组NO、TNF-α均低于模型组(P<0．05);中西药结合组N0、TNF-α均低于地塞米松组及热喘平口服液高、低剂量组(P<0．05).模型组,地塞米松组,中西药结合组及热喘平口服液高、低剂量组SOD均低于正常对照组(P<0．05),MDA均高于正常时照组(P<0．05);地塞米松组,热喘平口服液高、低剂量组及中西药结合组SOD均高于模型组(P<0．05),MDA均低于模型组(P<0．05);地塞米松组及热喘平口服液高、低剂量组SOD均低于中西药结合组(P<0．05),MDA均高于中西药结合组(P<0．05).结论 热喘平口服液平喘的作用机制与降低N0、TNF-α、MDA及升高血清中SOD有关.%Objective To approach the mechanism of Rechuanping oral liquid on experimental asthmatic guinea pigs. Methods 72 male guinea pigs were randomized into control group, model group, low dose Rechuanping oral liquid group, high dose Rechuanping oral liquid group, Dexamethasone group and integrated high dose Rechuanping oral liquid and Dexamethasone group. There were 12 guinea pigs in each group. Asthma models of guinea pigs were duplicated by egg albumin sensibilization. 0.9％ sodium chloride injection sprays instead of stimulation of egg albumin in control group. Low dose Rechuanping oral liquid group was given Rechuanping oral liquid [by 10 g/( kg · d) the equivalent of crude drug 3 g/d, made of 6 mL oral liquid]3 mL, twice intragastric administration a day. High dose Rechuanping oral liquid group was given Rechuanping oral liquid [by 20 g/( kg · d) the equivalent of crude drug 6 g/d, made of 6 mL oral liquid]3 mL, twice a day. Dexamethasone group was given Dexamethasone sodium phosphate injection [by 3.5 mg/(kg · d) the equivalentof Dexamethasone 1 mg/d, made of 6 mL suspensions]3 mL, twice a day. Integrated high dose Rechuanping oral liquid and Dexamethasone group was given combination of Rechuanping oral liquid [by 20 g/( kg · d) the equivalent of crude drug 6 g/d, made of 3 mL oral liquid]and Dexamethasone sodium phosphate injection [by 3.5 mg/( kg · d) the equivalent of Dexamethasone 1 mg/d, made of 3 mL suspensions]3 mL, twice a day. 0. 9％ sodium chloride injection was applied in control group and model group. The levels of Nitric oxide, TNF - α, SOD and MDA were detected in bronchoalveolar lavage fluid 7 days after intragustrie administration. Results The levels of NO and TNF - α in model group, low dose Rechuanping oral liquid group,high dose Rechuanping oral liquid group, Dexamethasone group and integrated high dose Rechuanping oral liquid and Dexamethasone group were higher than those in control group ( P ＜ 0. 05 ). The levels of NO and TNF - α in low dose Rechuanping oral liquid group, high dose Reehuanping oral liquid group, Dexamethasone group and integrated high dose Rechuanping oral liquid and Dexamethasone group were lower than those in model group ( P ＜ 0.05 ). The levels of NO and TNF - α in integrated high dose Reehuanping oral liquid and Dexamethasone group were lower than those in low dose Rechuanping oral liquid group, high dose Rechuanping oral liquid group and Dexamethasone group ( P ＜ 0.05 ). The level of SOD in model group, low dose Rechuanping oral liquid group, high dose Rechuanping oral liquid group, Dexamethasone group and integrated high dose Reehuanping oral liquid and Dexamethasone group was lower than that in control group ( P ＜ 0. 05). The level of MDA was higher than that in control group ( P ＜ 0.05).The level of SOD in low dose Rechuanping oral liquid group, high dose Rechuanping oral liquid group, Dexamethasone group and integrated high dose Rechuanping oral liquid and Dexamethasone group was higher than that in model group ( P ＜ 0.05 ). The level of MDA was lower than that in model group ( P ＜ 0.05 ). The level of SOD in low dose Rechuanping oral liquid group, high dose Rechuanping oral liquid group and Dexamethasone group was higher than that in integrated high dose Rechuanping oral liquid and Dexamethasone group ( P ＜ 0. 05). The level of MDA in low dose Rechuanping oral liquid group, high dose Rechuanping oral liquid group and Dexamethasone group was lowerthan that in integrated high dose Rechuanping oral liquid and Dexamethasone group ( P ＜ 0.05). Conclusion The mechanism of Rechuanping oral liquid is related to decrease of NO and TNF - α in bronchoalveolar lavage fluid of experimental asthmatic guinea pigs, increase of SOD of serum and decreased of MDA of serum. Integrated high dose Rechuanping oral liquid and Dexamethasone group Better effect on the treatment of asthma models of guinea pigs.