Objective To research the mechanism of a prescription for clearing turbid and poison on acute gouty arthritis rats .Methods 40 Wistar rats were randomly divided into blank group , model group , a prescription for clearing turbid and poison group and indometacin group , 10 rats in each group .Rats in blank group and model group received 0.9%sodium chloride injection 2 mL, once a day.Rats in a prescription for clearing turbid and poi-son group and indometacin group received a prescription for clearing turbid and poison and Indometacin Enteric -coa-ted Tablets soup 2 mL, once a day for four day .Acute gouty arthritis model rats in a prescription for clearing turbid and poison group and indometacin group were established by sodium urate suspension injected into the cavity ankle of rats.Rats in blank group and model group received the cavity ankle injection of 0.9%sodium chloride.After model-ing the original program continued gavage once daily , continuous 3 d.Comparison of body mass were observed before modeling and 72 h after modeling, gait changes and joint tissue matrix metalloproteinase (MMP-3) levels of content 1 h after the last administration were observed .Results Body mass 72 h after modeling was increased significantly only in blank group compared with that before modeling ( P<0 .01 ) .Compare the degree of change in body weightthat in model group (P<0.01).Joint tissue MMP -3 levels of content 1 h after the last administration in model group were increased compared with those in blank group (P<0.01).There was no significant difference between three groups on tissue MMP -3 levels (P>0.05).Conclusion A prescription for clearing turbid and poison can significantly improve gait and the overall function of acute gouty arthritis rats , and gastrointestinal side effects is small, its action mechanism is not obviously associated with lowering levels of MMP -3.%目的：观察清泻浊毒法对急性痛风性关节炎大鼠的作用，并探讨其机制。方法将40只Wistar大鼠随机分为空白组、模型组、清泻浊毒方组及吲哚美辛组，每组10只。空白组、模型组每日灌胃0．9％氯化钠注射液2 mL，清泻浊毒方组灌胃清泻浊毒方药液2 mL，吲哚美辛组灌胃吲哚美辛肠溶片药液2 mL，连续4 d。模型组、清泻浊毒方组及吲哚美辛组大鼠采用尿酸钠混悬液注入大鼠踝关节腔造成急性痛风性关节炎模型；空白组注入0．9％氯化钠注射液对照。造模后按原方案继续灌胃给药，每日1次，连续3 d。观察比较各组大鼠造模前及造模72 h后的体质量、步态变化及末次灌胃1 h后关节组织中的基质金属蛋白酶（MMP-3）水平含量。结果造模72 h后较造模前仅空白组体质量明显增加（P＜0．01），但从体质量增减程度比较，清泻浊毒方组分别与模型组及吲哚美辛组比较体质量增加明显（P＜0．01）；造模72 h后较造模前模型组、清泻浊毒方组及吲哚美辛组步态分级情况与正常组比较均有明显改变（P＜0．01），清泻浊毒方组及吲哚美辛组步态分级情况与模型组比较有明显改善（P＜0．01），且2组效果相当（P＞0．05）；模型组大鼠末次灌胃1 h后关节组织MMP-3水平较空白组比较明显增加（P＜0．01），清泻浊毒方组及吲哚美辛组与模型组比较MMP-3水平均有下降趋势，但比较差异无统计学意义（P ＞0．05）。结论清泻浊毒方能够明显改善急性痛风性关节炎大鼠的关节步态和整体功能，且胃肠道副作用小，其作用机制与降低MMP-3水平无明显相关。
展开▼
