目的：观察地黄和胃口服液对链脲佐菌素所致糖尿病大鼠糖耐量的改善作用。方法采用链脲佐菌素腹腔注射制作糖尿病大鼠模型。将50只糖尿病大鼠随机分为5组，每组10只，分别为模型对照组、地黄和胃口服液小剂量组（2．8 g生药/kg）、地黄和胃口服液中剂量组（5．6 g生药/kg）、地黄和胃口服液大剂量组（11．2 g生药/kg）及盐酸二甲双胍组（0．1 g/kg），另取10只正常大鼠作为正常对照组。分组后各药物组予相应药液1 mL/100 g体质量灌胃给药，正常对照组及模型对照组大鼠予等容积蒸馏水灌胃，连续7 d。第7 d给药30 min后予50％葡萄糖注射液2 g/kg灌胃，然后眶后静脉采血，测定0、0．5、1、2 h血糖值，计算血糖曲线下面积（ GAUC）。结果正常对照组及模型对照组大鼠葡萄糖灌胃后0．5 h血糖即达到高峰，且模型对照组大鼠0、0．5、2 h 3个时间点血糖值均高于正常对照组同期（P＜0．05），盐酸二甲双胍组大鼠及地黄和胃口服液大、中、小剂量组大鼠在0、0．5、2 h 3个时间点血糖值及GAUC均低于模型对照组同期（ P＜0．05）。结论地黄和胃口服液对糖尿病大鼠糖代谢有明显的改善作用，为临床应用提供了药效学依据。%Objective To observe the improvement of Dihuang Hewei oral Liquid on glucose tolerance of streptozotocin-induced diabetic rats .Methods 50 diabetic rats were randomly divided into model control group , low-dose Dihuang Hewei oral Liquid group (2.8 g crude drug /kg), middle-dose Dihuang Hewei oral Liquid group(5.6 g crude drug/kg), high-dose Dihuang Hewei oral Liquid group (11.2 g crude drug /kg) and Metform-in Hydrochloride group(0.1 g/kg).Another 10 normal rats was as control group.After grouping each drug group re-ceived the corresponding liquid 1 mL/100 g body weight administered orally , normal control group and model control group were given the same volume of distilled water for 7 d.After the first seven days after administration of 30 min 50% glucose 2 g/kg were given orally , then the orbital venous blood was collected and glucose values were measured at 0,0.5,1,2 h , the area under the glucose curve (GAUC) was calculated.Results Glucose peak of blood in control group and model control group was 0.5 h after administration.Blood glucose levels at 0,0.5,2 h of 3 time points in model control group were higher than those in normal control group ( P<0 .05 ) .Blood glucose levels and GAUC at 0,0.5,2 h of 3 time points in Metformin Hydrochloride group and low -, middle-, high-dose Dihuang Hewei oral Liquid group were lower than those in model control group (P<0.05).Conclusion Dihuang Hewei o-ral Liquid has improvement on glucose tolerance of streptozotocin -induced diabetic rats , and provides an evidence of pharmacodynamics for clinical application .
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