Objective To discuss the effects of hepcidin in proliferation, apoptosis, calcification function of osteoblasts. Methods Hepcidin hFOB1.10 was prepared and then made in vitro study. Prepare the medium of osteo-blasts' proliferation, apoptosis and calcification. Separately distribute 3 groups with control group, 100 nmol/L group with 100 nmol/L hepcidin intervention and 200 nmol/L group with 200 nmol/L hepcidin intervention. Proliferation was detected by MTT, apoptosis were stained by Biyuntian and detected by flow cytometry, calcium nodules counted by optical microscope after staining. Results The proliferative activity of 3 groups had no significant difference. The osteoblas apoptosis rate was gradually increased as hepcidin dose reduction, the data had significant difference (P<0.05). Calcium nodule count declined as hepcidin dose reduction, the data had significant difference (P<0.01). Conclusion Hepcidin may reduce the rate of osteoblast apoptosis, and enhance calcification, which had important reference value of prevention and treatment of osteoporosis.%目的 探讨铁调素在人成骨细胞增殖、凋亡以及钙化过程中的作用和影响.方法 成骨细胞hFOB1.10制备后行体外实验研究,分别进行成骨细胞增殖、凋亡和钙化形成的培养液配备,均分为三组:空白对照组、100 nmol/L铁调素干预的100 nmol/L组、200 nmol/L铁调素干预的200 nmol/L组.增殖活性采用MTT活检测,凋亡情况采用碧云天染色及流式细胞仪检测法,钙结节计数染色后采用光学显微镜观察.结果 三组成骨细胞增殖活性接近,经统计学比较差异无统计学意义(F=2.38,P＞0.05)；随着铁调素剂量的减少,成骨细胞凋亡率逐渐增高,数据经统计学比较差异具有统计学意义(F=12.28,P＜0.05)；随着铁调素干预的减少,钙结节计数呈下降趋势,三组成骨细胞钙结节计数经统计学比较差异具有统计学意义(F=18.28,P＜0.01).结论 铁调素可降低成骨细胞凋亡率、增强钙化形成,对于(进)一步研究骨质巯松的防治具有重要参考价值.
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