高效液相色谱法在静脉麻醉药血药浓度测定中的应用

摘要

高效液相色谱法(HPLC)是一种常用的测定血药浓度的方法,它具有灵敏度高、选择性好、定量准确等优点.HPLC法测定静脉非阿片类麻醉药(丙泊酚、咪达唑仑、依托咪酯等)、静脉阿片类麻醉药(吗啡、芬太尼、舒芬太尼等)和麻醉中的其他常用药物(顺式阿曲库铵、利多卡因等)血药浓度的结果显示,其具有较高的回收率、日内及日间精密度,适用临床血药浓度测定.%High-performance liquid chromatography (HPLC) is a commonly used method for the determination of plasma drug concentrations, which has the advantages of high sensitivity, good selectivity and accurate quantification. Determination by HPLC of the plasma concentration of intravenous non-opioid anesthetics (propofol, midazolam, etomi-date, etc.), intravenous opioid anesthetics (morphine, fentanyl, sufentanil, etc.) and other commonly used drugs in anes-thesia (cisatracurium, lidocaine, etc.) shows that it has a high recovery rate, intra-day and inter-day accuracy, and can be used for clinical plasma drug concentration determination.