O149大肠菌株的细菌学检测与肠毒素鉴定

摘要

By the analysis of epidemiology and pathogen,13 O149 Escherichia coli from piglet suffered from yellow dysentery were separated.Its producing-enterotoxin was tested by means of the adult rabbit ligated ileum loop assay and slide agglutination.The results of biological and serological test showed that the filtered solution of O149 Escherichia coli contained heat-sensitive enterotoxin.%通过流行病学及病原学的调查研究，从患黄痢的新生仔猪分离出13株O149大肠杆菌，采用兔肠结扎试验、平板免疫溶血试验对其产肠毒素性能进行了鉴定。生物学试验和血清学反应的结果表明，O149大肠菌株的被检滤液中存在热敏肠毒素（LT）。