菊芋叶黄酮类化合物的体外抗氧化活性研究

摘要

Scavenging ability of flavonoid from Helianthus tuberosus leaves on O2- · and · OH was determined by ultraviolet and visible spectrophotometer to explore its in vitro antioxidant activity. The results showed that the scavenging ability of flavonoid from Helianthus tuberosus leaves on O2-· and · OH increased with the increase of flavonoid concentration, and up to 66.45％ and 69. 33％ respectively when the flavonoid concentration was 100 μg/mL, Flavonoid from Helianthus tuberosus leaves also had good antioxidant activity on lard and could inhibit the increase of POV.%为探讨菊芋叶黄酮类化合物的体外抗氧化活性,采用分光光度法测定了菊芋叶黄酮类化合物对O2-·和·OH的清除能力,以及对猪油氧化的抑制作用.结果表明:随菊芋叶黄酮类化合物浓度的增加,其对O2-·和·OH的清除能力也相应增加.当菊芋叶黄酮浓度达100 μg/mL时,对O2-·的清除率达到66.45%,对·OH的清除率可达69.33%.此外,菊芋叶黄酮对油脂也表现出良好的抗氧化作用,可抑制猪油POV值的升高.