猪瘟和猪繁殖与呼吸综合征病毒混合感染的诊断

摘要

2009年3月贵州省某猪场送检病猪3例,为确诊病因,进行了流行病学调查,实验室剖检病变观察,细菌学、荧光抗体和RT-PCR检测.经流行病学调查和剖检病变观察,该病例为疑似猪繁殖与呼吸综合征病毒、猪瘟病毒感染所致;该病例细菌分离鉴定结果为阴性,猪瘟直接荧光抗体检测结果为阳性,猪繁殖与呼吸综合征病毒和猪瘟病毒RT-PCR核酸检测为阳性.表明,该病例为猪瘟病毒和猪繁殖与呼吸综合征病毒混合感染.%The pathogenesis of three sick pigs from the pig farm was determined by epidemiological survey, autopsy pathological observation; bacteriological detection, fluorescence antibody test and RTPCR. The results showed that three pigs may be infected by classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS), the bacterium isolated from three sick pigs was negative,the direct fluorescence antibody test of classical swine fever was positive, and the classical swine fever (CSF) and porcine reproductive and respiratory syndrome were positive by RT-PCR test, which indicates that three sick pigs were infected by virus of classical swine fever (CSF) and porcine reproductive and respiratory syndrome.