三七根部总 RNA 不同提取方法的比较

摘要

In order to obtain efficient extraction method and high quality RNA from P .pseudoginseng root,Comparison of Trizol method,CTAB method,modified Trizol method and isolation kit method for total RNA extraction from root of P .pseudoginseng was conducted.Results:The four methods were of various extraction effects,of which,the modified Trizol method with 2.00 OD260/OD280 ,1.90 OD260/OD230 ,1.9 28S∶ 18S,7.4 RIN,386 μg/μL mass concentration was the best.The other three methods easily lead to sample degradation or saltion residues.Conclusion:The modified Trizol method is an efficient extraction method of RNA from P .pseudoginseng root.%为探明三七根部总 RNA 的最佳提取方法，获取高质量 RNA，选取三七根部为材料，比较 Tr-izol 试剂法、CTAB 法、改良 Trizol 法和试剂盒法提取三七根部总 RNA 的质量。结果表明：4种方法提取三七根部 RNA 效果不同，其中以改良 Trizol 法提取 RNA 的质量最高，其 RNA 的 OD260／OD280为2．00， OD260／OD230为1．90，28S∶18S 为1．9，RIN 值为7．4，质量浓度为386μg／μL。而另外3种方法易出现样品降解、盐离子残留。结论：改良 Trizol 法适用于三七根部总 RNA 的提取。