基于SSR标记13个紫苏品种(系)的亲缘关系及遗传多样性

摘要

Genomic DNA fragments extracted from 13 P.frutescens varieties are amplified by 14 pairs of SSR primers and their genetic relationship and genetic diversity are identified and analyzed to provide a reference for identification and utilization of P.frutescens germplasm resources.Results: 1) 51 distinct DNA bands are identified in 13 P.frutescens varieties.47 bands in 51 bands are of polymorphism.2) The genetic distance and average genetic distance of 13 P.frutescens varieties is 0.086~0.576 and 0.336 respectively.The genetic similarity and average genetic similarity coefficient of 13 P.frutescens varieties is 0.424~0.914 and 0.654 separately.3) 13 P.frutescens varieties can be divided into two groups when the genetic distance is 0.59.Group I includes one Kaiyang local P.frutescens variety (CK) only and Group II includes 12 P.frutescens varieties of Qisu 1~6 and Yuanzhong 1~6.Group II can be divided into two subgroups when SM is 0.67.Subgroup I includes four P.frutescens varieties of Qisu 1, Qisu 6, Yuanzhong 1 and Yuanzhong 6 and Subgroup II includes eight P.frutescens varieties of Qisu 2~5 and Yuanzhong 2~5.When SM is 0.80, the genetic relationship among Yuanzhong 2, Yuanzhong 3 and Yuanzhong 4 is more close but the genetic relationship is at a distance after systemic breeding.%为紫苏种质资源利用提供参考,利用14对SSR引物对13个紫苏品种(系)提取的DNA进行PCR扩增,并对其亲缘关系进行鉴定与遗传多样性分析.结果表明: 1) 从13个紫苏品种(系)中共检测出51条清晰DNA条带,其中多态性条带47条,占92.16%.2) 13个紫苏品种(系)的遗传距离为0.086~0.576,平均遗传距离为0.336;遗传相似性系数为0.424~0.914,平均遗传相似性系数为0.654.3) 在遗传距离(SM)为0.59时,13个紫苏品种(系)可聚为2类群.类群Ⅰ为开阳紫苏(CK)1个品种(系),类群Ⅱ包括奇苏1~6号及其原种1~6号共12个品种(系).在SM为0.67时,类群Ⅱ又可分为2部分,第1部分包括奇苏1号和奇苏6号及其原种1号和原种6号共4个品种(系);第2部分包含奇苏2~5号及其原种2~5号共8个品种(系).在SM为0.80时,原种2号、原种3号和原种4号的亲缘关系最近,但经系选改良后,其亲缘关系已有一定距离.