采用高效液相色谱法和紫外分光光度法分别测定了不同生境中柔毛淫羊藿的有效成分,并运用oneway ANOVA统计分析了不同生境中柔毛淫羊藿有效成分的差异以及形态参数和生物量的差异.结果表明:3个生境中,相对光照强度较低的生境,其形态参数和植株各个部位的生物量均要低于光照强度相对较强的生境,但3个生境中的整体叶形基本一致；三个生境中淫羊藿总黄酮和淫羊藿苷含量在光照强度较高的生境中要大于光照较低的生境；柔毛淫羊藿植株各个部位的有效成分大小为叶＞根＞茎；在不同生境中柔毛淫羊藿叶片的黄酮和淫羊藿苷含量都能达到用药标准,其余的部位则不能达到用药标准.因此在人工栽培柔毛淫羊藿时,应模拟野外生长条件,以期提高柔毛淫羊藿质量,替代和保护野生资源.%Morphological characteristics and effective composition contents of Epimedium pubescens in different habitats were studied The effective composition contents were determined in different habitats by HPLC and UVand the difference of morphological parameters and effective composition contents were analysed using one-way ANOVA statistical analysis. The results indicated that morphological parameters and biomass were higher in high light intensity habitats than in low light intensity habitats, but the shape of leaves were same in three habitats. In the relatively high light intensity,the flavonoid and icariin contents of E. pubescens were significantly lower than the low light intensity habitats. The effective component in various parts of the E. pubescens was leaf>root>stenu According to the Chinese Pharmacopoeia,both the icariin and flavonoid contents only in E. pubescens leaves can meet the standard To improve the quality of Epimedium,substitute and protect of wild resources,we should simulate field growth conditions in artificial cultivation.
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