To date,there was no example to authenticate seed phospholipids based on their fingerprinting.By means of electrospray ionization mass spectrometry(ESI MS)and proton nuclear magnetic resonance(1 H NMR)spectroscopy,phospholipids fraction extracted from seeds of six species from the genus Michelia were detected.It was firstly found that distinct difference in spectral fingerprinting region between m/z 895-910 in ESI MS and 5.30-5.40 mg/L in 1 H NMR among these six species.Thus,it suggested that the spectral differences shown by ESI MS and 1 H NMR among seeds of these six species could be applied to identify them.And in a wider sense,for analyzing seed phospholipids of other plant species,a combination of ESI MS AND NMR was an effective tool.%首次尝试利用电喷雾电离质谱(ESI MS)和氢原子核磁共振(1 H NMR)技术分析了含笑属六种植物种子的磷脂特性,发现在两个指纹图谱区发现明显的差异,即在质荷比(m/z)895-910(ESI MS)和5.30~5.40mg/L(1 H NMR)两个特异的区域存在显著差异.这些源于种子磷脂ESI/MS和1 H NMR的谱带差异可以被用来分析不同植物的种子的磷脂特征.而且,相似地,在更广的层面上,特异性的谱带差异可用于分析其他植物种子的磷脂组成和特性,辅助鉴定种子.
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