利用高效液相色谱技术,对不同生长期的苦玄参样品进行分析,以揭示苦玄参苷类成分的积累动态规律.在对苦玄参植株生长情况进行观察的基础上,收集不同生长期的苦玄参样品,通过高效液相色谱法仪测定其中有效成分的含量,并进行色谱图比较及数据分析.结果表明:苦玄参总苷、苷IA和IB的含量随着生长周期的变化基本上呈现一致性,苦玄参苷IA和IB的含量均在75 d达到整个生理期的最高峰,随后缓慢下降,但在果期前期(即120 d)两者含量再次出现次高峰,此时的生物量达到最大.由此可以确定苦玄参药材最佳采收期应在果期前期进行采收.%The content of glycosides of P. felterrae in different growth period were detected with HPLC to reveal its dynamic accumulation. The correlation analysis of glycosides and the growth situation was conducted throught comparing the chromatograms and peak areas of different growth period. The results showed that the variation trend of content of glycosides, picfeltarraenin IA and Ptarraenin IB was nearly consistent. The content of picfeltarraenin IA and Ptarraenin IB both reached to the maximum when the plant grew up to 75 days, then subsequently decreased slowly,but increased again in early fruit stage(namely 120d)when the biomass reached to the maximum. Therefore, it was suggested that the best collection stage for P. felterrae was the early fruit stage(120 d).
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