微量元素营养对桉树无性系苗木叶绿素及荧光参数影响

摘要

The effects of level and form of trace elements on chlorophyll and its fluorescence in seedling leaves from 3 eu-calypt clones were studied by way of sand culture.The results showed that the chlorophyll content and fluorescence pa-rameters were influenced by factors significantly.The eucalypt clone was an important effective factor and had high sig-nificantly influence on all traits.In the factors of trace elements,there existed high significant differences in Fe(B)to Fo, Fm,Fv/Fo and Fv/Fm,Zn(C)to Fo and Fv/Fm,Mn(D)to chlorophyll content(SPAD value),Mo(F)to chlorophyll content and Fo,B(G)to chlorophyll content,Fo,Fv/Fo and Fv/Fm,respectively.There were also significant relation-ships between Fm and Fo,between chlorophyll content and Fo,Fm,Fv/Fm and Fv/Fo.With the increase of Fe,Zn,Cu, Mo,B solution nutrition,Fo and Fv/Fm increased,but Fv/Fm decreased under the higher concentration.Compared with chlorophyll fluorescence,U6 and DH32-29 had higher Fo,Fm value and lower Fv/Fm,Fv/Fo value,but DH32-29 was more susceptible to abundance and deficiency of trace elements in soil.The results also showed that applying chlorophyll fluo-rescence would be a good method to evaluate the health of eucalypt species in trace element nutrient.%研究了不同微量元素营养液,对栽培的三种桉树无性系苗木叶绿素及荧光参数变化.结果表明：各无性系苗木在全部测试性状的差异表现出极显著.在六个测试的微量元素因子中,Fe(B)显著地影响苗木的Fo、Fm、Fv/Fo、Fv/Fm,Zn(C)显著地影响Fo、Fv/Fm,Mn(D)显著地影响叶绿素含量(SPAD值),Mo(F)显著地影响叶绿素含量、Fo,B(G)显著地影响叶绿素含量Fo、Fv/Fo、Fv/Fm.相关分析表明Fm与Fo、Fv/Fo与Fo、叶绿素含量与Fo、Fm、Fv/Fm与Fv/Fo均存在显著的相关性.随着微量元素 Fe、Zn、Cu、Mo、B浓度增大,Fo、Fv/Fm均呈增大趋势,但在不利的高浓度环境下,Fv/Fm降低.对三个无性系叶绿素荧光比较,U6与DH32-29具有高的Fo、Fm和低的Fv/Fm、Fv/Fo,DH201-2却具有低的Fo、Fm和高的Fv/Fm、Fv/Fo.对微量元素浓度变化,DH32-29较敏感.因此,叶绿素荧光参数可作为诊断植物微量元素营养状况的指标之一.