黄枝油杉遗传多样性的ISSR分析

摘要

ISSR markers were used to investigate the genetic diversity of seven natural populations of Keteleeria calcarea. Twelve ISSR primers produced 125 loci. At the species level, the percentage of polymorphic loci (PPL) was 100.00%, Shannon’s Information index (I) was 0.417 7 and Nei’s gene diversity index (H) was 0.266 6. At the population level, the percentage of polymorphic loci was 71.20%-92.00%and the average value was 80.69%, Shannon’s Information index (I) was 0.327 3-0.388 6 with the average value 0.3548 and Nei’s gene diversity index (H) was 0.213 9-0.247 8 with the average value 0.229 1. K. calcarea in both species and population levels showed higher genetic diversity. Nei’s genetic diversity analysis (Gst=0.143 3) and AMOVA analysis (Φst=17.91%) showed that the genetic variation of K. calcarea mainly occurred within populations, and the genetic differentiation among populations was low. The gene flow ( Nm) be￣tween populations was high (2.989 0). Mantel analysis showed that the genetic distance of K. calcarea natural populations had no significant correlation with their geographic distance ( r=0.456 7, P=0.061 0>0.05) .%该研究采用ISSR分子标记,对黄枝油杉7个自然种群的遗传多样性进行了分析。结果表明：用12条ISSR引物对218个黄枝油杉个体进行扩增,共扩增出125个位点。在物种水平上,多态性位点百分数( PPL)为100.00％,Shannon信息多样性指数( I)为0.4177,Nei’ s基因多样性指数( H)为0.2666；在种群水平上,多态性位点百分数(PPL)在71.20％~92.00％之间,平均值为80.69％,Shannon信息多样性指数(I)在0.3273~0.3886之间,平均值为0.3548,Nei’ s基因多样性指数( H)在0.2139~0.2478之间,平均值为0.2291。这说明黄枝油杉在物种水平和种群水平上均显示出较高的遗传多样性。 Nei’ s遗传多样性分析( Gst＝0.1433)和AMOVA分析(Φst＝17.91％)表明,黄枝油杉的遗传变异主要存在于种群内,种群间的遗传分化程度较低,种群间保持一定的基因交流( Nm＝2.9890>1)。 Mantel分析显示,黄枝油杉种群间的遗传距离和地理距离之间不存在显著的相关关系( r＝0.4567, P＝0.0610>0.05)。