广西产拳卷地钱HPLC指纹图谱研究

摘要

In order to control the quality of Marchantia convoluta,we adopted HPLC fingerprint method to study eight batches of medicinal materials. The separation was developed on ZORBAX SB-C18(4.6 mm × 250 mm,5 μm)by gradient elution with acetonitrile-water(both containing 0.1% phosphoric acid)as mobile phase at a flow rate of 1.0 mL·min-1,at the column temperature of 25℃ and with the detection wavelength of 230 nm.The reference fingerprints were established,and six common peaks were found.The similarity of HLPC fingerprints of eight batches of M.convoluta was greater than 0. 75. The precision, repeatability and stability of the experiments were in line with the requirements. The data of HPLC fingerprint analysis are stable and reliable. The method is simple and efficient,which can provide reference for the quality control of M. convoluta.%为了对广西产拳卷地钱药材进行质量控制,采用HPLC指纹图谱法对8批药材进行了研究,色谱条件为安捷伦ZORBAX SB-C18色谱柱(4.6 mm × 250 mm,5 μm),流动相为乙腈(B)-0.1%磷酸水溶液(D),梯度洗脱,检测波长为230 nm,柱温为25 ℃.结果表明:建立了参考指纹图谱,共有峰6个,8批广西产拳卷地钱药材HLPC指纹图谱相似度均大于0.75,其精密度、重复性、稳定性实验均符合指纹图谱技术要求.HPLC指纹图谱分析,数据稳定可靠,方法简便高效,可为广西产拳卷地钱质量评价提供参考.