HPLC法测定贞芪扶正薄膜衣片中齐墩果酸的含量

摘要

Objective To determine the contents of the Zhenqifuzheng film-coatd tables by HPLC. Methods The HPLC method was established for the content determination of oleanolic acid in the Zhenqifuzheng film-coatd tables,was used with a mobile phase consisting of acetonitrile-methanol-water-ammonium acetate(70:16:14:0.5) ,the flow rare was 1.0 mL/min,the detection wavelength was 215 nm,and the column tempreture was room temperature. Results A good linearity was obtained in the range of 0.00824~2.06mg/mL (r=0.9999) , linear equation is y=9905.62+2012.30x ,the average recovery was 99.57% with RSD of 1.01%(n=5).  Conclution The established method is simple,fast,reliable,accurate, and can be used as quality control of the Zhenqifuzheng film-coatd tables.%　　目的 建立贞芪扶正薄膜衣片的含量测定方法.方法 采用高效液相色谱法测定贞芪扶正薄膜衣片中齐墩果酸的含量.流动相：乙腈-甲醇-水-乙酸铵(70∶16∶14∶0.5)；流速为1.0mL/min,检测波长：215nm；柱温为室温.结果 在0.00824~2.06mg/mL范围内线性关系良好(r=0.9999),线性方程为y=9905.62+2012.30x.平均回收率为99.57%,RSD=1.01%(n=5).结论 所建立的方法简便、快速,结果可靠、准确,可用于贞芪扶正薄膜衣片的质量控制.