目的探寻超声内镜引导下的缓释吉西他滨组织间化疗在猪的胰腺的安全性和可行性。方法在超声内镜引导下将19G穿刺针刺入猪的胰腺组织中，通过穿刺针将能够缓慢释放吉西他滨的化疗粒子植入至胰腺组织的特定位置。观察14d后，处死动物取出放置粒子部位的胰腺组织进行观察。结果所有化疗粒子都被成功植入，没有粒子发生移位。胰腺组织发生局限的纤维素样坏死区域。在病灶中心1cm的范围内凋亡细胞数量增多。结论超声内镜引导下植入吉西他滨用于胰腺的组织间化疗不仅安全、耐受性好，而且可使组织消融、诱导细胞凋亡。%Objective To investigate the feasibility and safety of endoscopic ultrasonography(EUS)guided interstitial chemotherapy for the pancreas in pig’ s mode1. Methods A therapeutic 19-gauge needle was inserted into the pancreas with EUS guidance, and seeds with sustained release of gemcitabine were implanted into the tissue. After 14 days of clinical observation, the animals were sacrificed and the tissue response to local chemotherapy was examined. Results All the seeds were implanted successfully and no migration was observed. Chemotherapy induced tissue fibrous necrosis was localized in the pancreas without significant complications.The apoptotic index of tissue within 1 am of the seeds increased. Conclusion Implanted gemcitabine into the pancreatic tissue with the guiding of ultrasound endoscopy is not only safety, but also tolerability, it can make the tissue ablate, induce the cell apoptosis.
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