参附注射液对兔供心的保护作用

摘要

目的探讨参附注射液（SFI）能否提高供心的保护作用。方法16只成年新西兰大白兔（雄性），在Langendorff 离体灌注模型上，模拟心脏移植的“供心获取前心脏停跳”、“供心冷保存”、“主动脉开放后供心复灌”三个时期。实验组：供心离体灌注装置上平衡后，以含 SFI 的4℃冷 Stanford 液灌注诱导停跳；之后在含 SFI 的4℃冷 Stanford液中冷浸浴原位保存4小时；保存结束后，开放主动脉重新开始灌注复苏；对照组：全程不用 SFI（用生理盐水替代 SFI），余同实验组。测定两组心脏血流动力学、冠脉流量、心肌梗死量、心肌组织含水量。结果使用 SFI 的实验组，心脏血流动力学和冠脉流量优于对照组（P ＜0.05），而心肌组织梗死量和心肌含水量低于对照组（P ＜0.05）。结论参附注射液能减轻供心“缺血-再灌注”引起的心肌损伤，显著提高供心的保护效果。%Objective Previous studies have demonstrated that Shenfu injection (SFI)could protect donor heart against post-ischemia injury when applied immediately after reperfusion,but its potential cardioprotective effect added to Stanford Solu-tion (STF)prior to heart arrest and during the cold heart preservation is unknown. Methods Sixteen rabbits were anesthe-tized.Hearts were harvested and subjected to Langendorff perfusion.After equilibrium,hearts were arrested with a cold Stanford solution (STF),followed by 4 hours of cold statistic preservation in STF,and 90 min reperfusion subsequently.SFI (60ml/L) were added to STF solution separately in above mentioned periods in experiment group (SFI-STF,n =8),where saline vehicle were added to STF in control group (Vehicle-STF,n =8). Results Myocardial water content and infarct size were significant-ly decreased in SFI-STF as compared with Vehicle-STF (P <0.05).Cardiac function in SFI-STF hearts returned to satisfactory level shortly after the initiation of reperfusion and still better preserved throughout the reperfusion;however,vehicle-STF hearts showed persistent hypokinesia during the peri-reperfusion period. Conclusion The addition of SFI (60ml/L)to the STF in-duced an improvement in functional recovery,alleviate ischemia-reperfusion induced myocardial injury,and afford cardioprotec-tive role for donor hearts protection.