HPLC-ESI-MS^2法测定制药废水中青霉素G残留

摘要

A method was developed for the simultaneous determination of PEN G in pharmaceutical wastewater by high performance liquid chromatography-electrospray tandem mass spectrometry（HPLC-ESI-MS2）.The sample was purified by Octadecyl C18 solid-phase extraction column.The high performance liquid chromatograph（HPLC） separation was performed with acetonitrile-water-acetic acid（volume ratio 20：80：0.005） as mobile phase.The identification and quantification were done with mass spectrometry.The limit of detection was 5 μg/L.The recovery ranged from 64.9% to 76.3% with relative standard deviation 6.2%.The experimental results showed that this method was accurate and rapid,which can be used for the residue analysis of PEN G in pharmaceutical wastewater.%建立了一种测定制药废水中青霉素G残留的高效液相色谱-电喷雾质谱方法。样品经Octadecyl C18固相萃取柱净化后,以乙腈-水-乙酸（体积比20∶80∶0.005）体系溶液作为流动相,电喷雾质谱检测器进行定性和定量分析。该方法检出限为5μg/L,相对标准偏差（RSD%）为6.2%,回收率是64.9%～76.3%。实验结果表明,该方法具有准确、快速的特点,适合制药废水中青霉素G残留的测定。