抗衰老保健食品中白藜芦醇和辅酶Q_（10）的含量测定

摘要

建立了同时测定抗衰老保健食品中白藜芦醇和辅酶Q10的含量的HPLC方法。采用Welch materials C18液相色谱柱,甲醇∶异丙醇（55∶45）为流动相,流速设定为1 mL·min^-1,检测波长为PDA定时波长（0.00,306.0）（6.00,306.0）（6.01,275.0）（20.00,275.0）,实现了白藜芦醇和辅酶Q10的良好分离分析。白藜芦醇的线性范围为0.52～156.00μg·mL^-1,r=0.9999;辅酶Q10的线性范围为0.49～195.52μg.mL^-1,r=0.9999。平均加标回收率及RSD分别为97.70%（RSD 0.6%）和97.40%（RSD 0.4%）。本方法灵敏度高、选择性好、操作简单,可方便地用于检测抗衰老类保健食品中白藜芦醇和辅酶Q10的含量。%A high performance liquid chromatographic（HPLC） method was established for the simultaneous determination of resveratrol and Coenzyme Q10 in anti-aging health-care food.The determination of resveratrol and Coenzyme Q10 by HPLC was performed with Welch materials ODS C18 and the mobile phase of methanol：isopropanol（55：45） at a flow rate of 1.0 mL·min^-1,and the detection wavelength was conducted at（0.00,306.0）,（6.00,306.0）,（6.01,275.0）,and（20.00,275.0）.Results showed that a good separation of resveratrol and Coenzyme Q10 was observed.The calibration curve of resveratrol was linear within the range of 0.52~156.00 μg·mL-1 and r=0.9999.The calibration curve of Coenzyme Q10 was linear within the range of 0.49~195.52 μg·mL^-1 and r=0.9999.The average recovery rate of resveratrol was 97.70% and the RSD was 0.6%.The average recovery rate of Coenzyme Q10 was 97.40% and the RSD was 0.4%.The HPLC method was characterized by high sensitivity,good selectivity,simple operation of determination of resveratrol and Coenzyme Q10 in anti-aging health-care food.