在单因素实验的基础上,采用Box-Behnken中心组合实验和响应面分析法,优化了皖南红豆杉枝叶中多糖的微波提取工艺。研究了提取时间、微波功率、料液比和浸泡时间对红豆杉枝叶多糖提取率的影响,建立了影响因素与响应值之间的数学模型,确立最佳提取工艺。结果表明:皖南红豆杉枝叶中多糖的最佳提取工艺为:微波提取6.11 min,液料比为30砄1,浸泡时间为119.21 min,微波功率为6 kW。该方法为提高皖南红豆杉枝叶中多糖成分的提取提供了基础。%The study aimed to get optimized craft of polysaccharide extraction from Taxus wallichiana var.mairei in Southern Anhui in China.On the basis of single-factor experiments, then using Box-Behnken design and its response surface analysis , the main factors of extraction time , power of microwave , the ratio between solvent and material and soaking time affecting the extraction , effects of saccharide were studied and mathematical model between the response value and factors was established.The optimal craft was as follows:30 times of water , extracting 6.11 min by 6 kW power of microwave.The design and results provided the theoretical foundation of production process .
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