为了建立盐酸莫西沙星葡萄糖注射液中依地酸钙钠的含量测定方法,采用反相离子对色谱测定方法,以Dikma Diamonsil C8为色谱柱(250 mm×4.6 mm,5 μm),缓冲液为流动相A,甲醇为流动相B,进行梯度洗脱,流速1.2 mL·min-1,柱温40 ℃,检测波长254 nm,进样量100 μL.检测结果表明:依地酸钙钠浓度在0.00504~2.016 μg·mL-1范围内与峰面积呈良好的线性关系,r=0.9999,平均加样回收率为100.7%.该方法快速、简便,专属性强,重复性好,结果准确可靠,适用于盐酸莫西沙星葡萄糖注射液中依地酸钙钠的含量测定.%A detection method of the calcium disodium edetate in moxifloxacin hydrochloride and glucose injection was established, using reverse phase ion chromatography, Diamonsil C8 column (250 mm×4.6 mm, 5 μm), buffer as mobile phase A, methanol as mobile phase B, gradient elution, flow rate of 1.2 mL·min-1, the column temperature of 40 ℃, the detection wavelength was 254 nm and 100 L sample volume.The results showed that the concentration of sodium calcium in the range from 0.00504 g·mL-1 to 2.016 g·mL-1 was in good linear relationship with the peak area, r=0.9999, and the average recovery rate was about 100.7%.This method was rapid, simple, specific, reproducible, accurate and reliable
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