探索甘草在单药、甘草-甘遂对药、甘遂半夏汤不同配伍中及炮制前后甘草酸的含量变化.采用 HPLC 法, Sinochrom C18反相色谱柱(4.6 mm×200 mm, 5 μm),以乙腈-0.05%的磷酸溶液(36:64)为流动相,流速为1.0 mL/min,柱温为室温,检测波长为250 nm.实验得出的甘草酸在甘草-甘遂对煎中含量最高,其次是甘草单煎液中,而在甘遂半夏汤中甘草酸的含量却是最低的.甘草经蜜制后,甘草酸铵的含量较生甘草含量有所下降.%Glycyrrhizic acid content changes of licorice in single medicine, licorice and euphorbia kansui on medicine, euphorbia kansui, pinellia decoction in different compatibility and before and after processing were explored. HPLC method,the sinochrom C18 reversed phase chromatographic column(4.6 mm×200 mm,5 μm),with acetonitrile-0.05% phosphoric acid solution (36:64) was used as the flow phase, the flow rate was 1.0 mL/min, the column temperature was room temperature,and the detection wavelength was 250 nm. The results showed that glycyrrhizin was the highest in glycyrrhizin, followed by licorice single decoction, and the content of glycyrrhizic acid was the lowest. The content of ammonium glycyrrhizinate decreased with the content of glycyrrhizae.
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