Objective To observe the effects of Sarcandrae Compound on proliferation and telomerase activity in HepG2 cells. Methods Human liver cancer HepG2 cells were treated with different concentrations of Sarcandrae Compound for 48 hours. Morphologic changes of cells were observed. MTT assay was used to evaluate the effects of different concentrations of Compound Sarcandrae on the proliferation of HepG2 cells and calculate IC50. ELISA was applied to detect the telomerase activity of HepG2 cells inhibited by Sarcandrae Compound and Paclitaxel. Results After HepG2 cells were treated with Sarcandrae Compound for 48 hours, cells fell off obviously and proliferation activity reduced significantly( P <0.05 ),which presented a dose-dependent trend. IC50 was 292.2 mg/ml. Sarcandrae Compound could significantly decrease the telomerase activity of HepG2 cells as well as Paclitaxel( P <0.05 ),and their inhibitory effects were similar( P >0. 05 ). Conclusion Sarcandrae Compound can significantly inhibit the proliferation of HepG2 cells and their telomerase activity.%目的 探讨肿节风复方对人肝癌细胞HepG2细胞增殖及端粒酶活性的影响.方法 以不同浓度肿节风复方处理肝癌HepG2细胞48 h,观察细胞形态学改变;采用MTT法评价不同浓度肿节风复方对HepG2细胞增殖的影响并计算半抑制浓度(IC50);应用ELISA法检测肿节风复方、紫杉醇对HepG2细胞端粒酶活性的影响.结果 肿节风复方处理HepG2细胞48 h后,细胞脱落明显,增殖活力显著降低(P＜0.05),呈现剂量依赖趋势;肿节风复方对HepG2细胞IC50为292.2 mg/ml.肿节风复方和紫杉醇对HepG2细胞端粒酶活性均有抑制作用(P＜0.05),并且抑制作用相当(P＞0.05).结论 肿节风复方能抑制HepG2细胞增殖及端粒酶活性.
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