薯蓣皂苷对K562细胞的细胞毒作用及对DNA影响的研究

摘要

目的 研究薯蓣皂苷的细胞毒作用和对遗传物质DNA损伤效应.方法 利用MTT法测定薯蓣皂苷对白血病细胞株(K562细胞)的体外抑制作用,用彗星实验法检测薯蓣皂苷元对K562细胞的DNA损伤.结果 薯蓣皂苷对K562细胞的生长有抑制作用,有量效关系.薯蓣皂苷可以导致K562细胞DNA发生断裂,随薯蓣皂苷的浓度梯度增大(1～500 μg/ml),细胞的拖尾率、DNA尾部百分率、尾长、尾矩和Olive尾矩增加.结论 薯蓣皂苷元对K562细胞的生长具有抑制作用,并会引起DNA断裂,可能会引起细胞DNA损伤,或者诱导细胞凋亡.%Objective To study the effect of dioscin on cytotoxicity and DNA damage of K562 cells. Methods MTT assay was used to determine the inhibition of dioscin on leukemia cell lines( K562 cell ) in vitro, and DNA damage of K562 cells was detected by Comets experiment. Results K562 cells growth was inhibited by dioscin in a dose-effect manner,and dioscin could catalyze the DNA cleavage of K562 cells. The comet tail rate of cells,the percentage of DNA in the tail, tail length, tail moment and Olive tail moment increased with dioscin concentration gradient increased( 1 - 500 (μg/ml). Conclusion Dioscin can inhibit K562 cells growth, and cause DNA cleavage, which may lead to DNA damage or induce cells apoptosis.