相思藤总黄酮对小鼠急性化学性肝损伤的保护作用及机制

摘要

目的 研究相思藤总黄酮(XSTF)对四氯化碳(CCl4)、对乙酰氨基酚(AP)和D-半乳糖胺(D-GalN)所致小鼠急性化学性肝损伤的保护作用,并探讨其作用机制.方法 分别以CCl4、AP和D-GalN诱导小鼠急性肝损伤为模型,检测其血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)以及肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)和谷胱甘肽过氧化酶(GSH-PX)的含量,观察XSTF对急性化学性肝损伤的影响.结果 XSTF明显降低CCl4、AP及D-GalN所致急性肝损伤小鼠的AST、ALT活性,并呈现明显的量效关系;XSTF能显著提高肝组织中SOD、GSH和GSH-PX含量,并显著降低MDA含量(P＜0.05).结论 相思藤总黄酮对小鼠急性化学性肝损伤具有一定的保护作用,可能与其清除自由基,抑制脂质过氧化有关.%Objective To investigate the protective effect and mechanism of XIANGSITENG flavonoid ( XSTF ) on CC14, AP and D-Galactosamine( D-GalN )-induced acute chemical liver injuries in mice. Methods The models of CC14 ,AP and D-GalN acute chemical liver injury in mice were used to study the effects of XSTF on acute chemical liver injury. The activities of ALT, AST in serum and the content of SOD, MDA, GSH and GSH-PX in hepatic tissue were measured. Results XSTF could significantly decrease the elevation of AST or ALT in liver injuries induced by CC14, AP or D-GalN in mice, which showed a does-effect relationship obviously. XSTF could obviously promote the content of SOD,GSH and GSH-PX in liver tissue,and decrease the MDA content significantly( P <0. 05 ). Conclusion XSTF could protect mice from CC14 ,AP and D-GalN-induced acute chemical liver injuries and its mechanisms may be related to attenuating free radical and inhibiting the lipid peroxidation.