小鼠肾发育中的细胞凋亡研究

摘要

Objective To investigate the cell apoptosis in kidney development in mice and related morphological characteristics.Methods In the resin sections,light microscopy and in situ end labeling(TUNEL) were applied to observing embryos and postnatal apoptosis of renal cells in mice of different ages .Results The cell apoptosis was found in embryonic kidney ureteric bud of 12-day-old mice, the cortical apoptosis cells mostly appeared between S bodies and renal corpuscle body in nephrogenic zone ,the apoptosis peak time of renal corpuscle in the cortex was embryonic age of 14-18 days.The renal tubules,medullary rays and medulla apoptotic cells were found in the renal tubular epithelium,their apoptosis peak appeared on the 7th day after birth.The observation on cortex and medulla apoptotic cells showed that the cells were phagocytized by neighboring cells,or partially exfoliated into the tubular lumen.Conclusion The apoptosis exists during the development of kidney in mice,which does exist in the development of metanephros.The apoptosis in renal cortex correlates with nephrogenic zone and improved renal corpuscle , the apoptosis in the medulla correlates with the reconstruction and improvement of renal tubule and collecting duct in the medulla .%目的：探讨小鼠肾脏发育过程中的细胞凋亡规律及相关形态学特征。方法在树脂切片上，应用光镜技术和原位末端标记法（ TUNEL ）分别观察不同胚龄及生后不同日龄小鼠肾脏细胞凋亡。结果胚龄12 d的小鼠肾脏输尿管芽中即存在细胞凋亡现象，皮质凋亡细胞多出现在生肾区S小体之间和肾小体内，皮质中的肾小体凋亡高峰期在胚龄14～18 d。而肾小管、髓放线及髓质凋亡细胞出现在肾小管上皮内，三者的凋亡高峰期均出现在生后7d左右。观察发现皮质和髓质凋亡细胞被邻近细胞吞噬，或部分脱落到肾小管腔内。结论小鼠肾脏发育过程中存在细胞凋亡，凋亡现象在后肾形成时即存在；皮质中细胞凋亡与生肾区的出现和肾小体发育完善有关，髓质中的细胞凋亡则与髓质中肾小管和集合管的改建、完善有关。