短期乙醇暴露后雌性大鼠肝功能酶类指标的动态分析

摘要

Objective To investigate the dynamic changes of liver function enzymes of short-term ethanol-exposed female rats.Methods Forty healthy adult female rats were randomly divided into control group ,low-dose ethanol group, middle-dose ethanol group and high-dose ethanol group,with 10 rats in each group.The rats in the control group were given sterile distilled water ,while in the low-,middle-and high-dose ethanol groups were given 7.893 g/L,15.786 g/L and 23.679 g/L ethanol,respectively.The mice were treated by intraperitoneal injection ,with the dose of 5 ml/kg,once a day for 4 weeks.The activities of alkaline phosphatase(ALP),lactic dehydrogenase(LDH),glutamic-pyruvic transaminase (ALT),glutamic-oxalacetic transaminease (AST) and cholinesterase (CHE) in serum were detected.Results With the increasing time of ethanol exposure,the activity of serum ALT increased (P<0.05),the activity of serum AST increased and then decreased in different doses ethanol groups (P<0.05).The activity of serum LDH increased and then decreased with the increasing time of ethanol exposure ,the activity of serum LDH in the high-dose ethanol group was significantly lower than that in the control group four weeks after ethanol exposure (P<0.05).With the increasing time of ethanol exposure ,the activity of serum ALP decreased in the control group , low-and middle-dose ethanol groups , but decreased and then increased in the high-dose ethanol group ,the activity of serum ALP in the high-dose ethanol group was significantly higher than that in the control group four weeks after ethanol exposure (P<0.05).With the increasing time of ethanol exposure ,the activity of serum CHE increased in the control group ,low-and middle-dose ethanol groups ,but decreased in the high-dose ethanol group,the activity of serum CHE in the high-dose ethanol group was significantly lower than that in the control group in the second and fourth weeks after ethanol exposure (P<0.05).Conclusion The serum enzymes of sensitivity of female rats with liver injury induced by ethanol exposure were CHE ,LDH and ALP,in which CHE is more sensitive , they all show dynamic changes .%目的：探讨短期乙醇暴露后雌性大鼠肝功能酶类指标的动态变化。方法将40只健康成年雌性大鼠随机分为对照（无菌蒸馏水）组和低剂量（7．893 g/L）、中剂量（15．786 g/L）、高剂量（23．679 g/L）乙醇染毒组，每组10只。采用腹腔注射方式进行染毒，染毒量为5 ml/kg，1次/d，连续染毒4周。检测大鼠血清碱性磷酸酶（ ALP）、乳酸脱氢酶（ LDH）、谷氨酸氨基转移酶（ ALT）、天门冬氨酸氨基转移酶（ AST）和胆碱酯酶（CHE）活性。结果随着乙醇暴露时间的延长，不同浓度乙醇组大鼠血清ALT活性均呈上升趋势（P＜0．05）；不同浓度乙醇组血清AST活性均呈先升高后降低的趋势（P＜0．05）；血清LDH活性随着乙醇暴露时间的延长呈先升高后降低的趋势，在乙醇暴露第4周时，高剂量组血清LDH活性明显低于对照组（ P＜0．05）；随着乙醇暴露时间的延长，对照组和低剂量组、中剂量组血清ALP活性均呈下降趋势，而高剂量组血清ALP活性呈先降低后升高的趋势，在乙醇暴露第4周时，高剂量组血清ALP活性明显高于对照组（ P＜0．05）；随着乙醇暴露时间的延长，对照组、低剂量组、中剂量组血清CHE活性均呈上升趋势，而高剂量组血清CHE活性则呈下降趋势，在乙醇暴露后第2、4周时，高剂量组血清CHE活性均明显低于对照组（ P＜0．05）。结论乙醇暴露导致雌性大鼠肝损伤，其敏感血清酶指标为CHE、LDH及ALP，且CHE更敏感，均呈动态变化。