急性痛风性关节炎大鼠P2Y6的表达水平及意义

摘要

Objective To explore the expression level of P2Y6 and its significance in rats with acute gouty arthritis. Methods Thirty male SD rats were randomly divided into control group,model group and colchicine group,with 10 rats in each group. The colchicine group was given colchicine by gavage,and the control group and the model group were given the equivalent amount of normal saline by gavage. On the third day after intragastric administration,monosodium urate(20 mg/ml) was injected into right ankle of the rats in the model group and colchicine group,50 μl per rat,while the injection of the equivalent amount of normal saline was performed in the control groups. Then to stop intragastric administration on the fifth day. The swollen joint count index,pathological changes of joint cartilage,and expressions of P2Y6 mRNA and protein in ankle tissues were compared among the three groups after modeling. Results The swollen joint count indices in the model group were higher than those in the other two groups at 4 h,12 h,24 h and 48 h after molding(P<0. 05),and at 12 h and 24 h after modeling,the swollen joint count indices in the colchicine group were higher than those in the control group but lower than those in the modeling group(P<0. 05). Microscopic findings revealed that obvious inflammatory cell infiltration and active proliferation of cartilage cells were observed in ankle tissues of the model group,but the features above were milder in the colchicine group. The expression levels of P2Y6 mRNA and protein increased in turn in the normal group,the colchicine group and the model group(P<0. 05). Conclusion Elevated P2Y6 level is seen in the rats with acute gouty arthritis. P2Y6 might play an important role in the incidence of acute gouty arthritis, and it might be a new therapeutic target.%目的 探讨急性痛风性关节炎大鼠P2Y6的表达水平及意义.方法 将30只雄性SD大鼠随机分为对照组、模型组、秋水仙碱组各10只,秋水仙碱组给予秋水仙碱灌胃,对照组、模型组以等量的生理盐水灌胃,第3天灌胃后,模型组、秋水仙碱组大鼠用20 mg/ml尿酸钠溶液注射右踝关节,50μl/只,对照组注射等量生理盐水,继续灌胃至第5天.比较造模后3组大鼠关节肿胀指数、关节软骨病理变化以及踝关节组织P2Y6 mRNA和蛋白的表达情况.结果 模型组大鼠造模后4 h、12 h、24 h、48 h踝关节肿胀指数高于其他两组(P<0.05),秋水仙碱组在造模后12 h、24 h踝关节肿胀指数高于对照组但低于模型组(P<0.05).镜下可见模型组踝关节组织存在明显炎症细胞浸润,软骨细胞增生活跃,而秋水仙碱组的表现较经.对照组、秋水仙碱组、模型组大鼠踝关节P2Y6 mRNA及蛋白表达水平依次增高(P<0.05).结论 急性痛风性关节炎大鼠P2Y6水平高,P2Y6在急性痛风性关节炎的发生中可能起到重要作用,其或可成为治疗新靶点.