铁皮石斛益胃作用的谱效关系

摘要

目的 探讨铁皮石斛的高效液相色谱(HPLC)指纹图谱与益胃作用间的关联性.方法 取4个产地铁皮石斛,分别制备水提取物和醇提取物及其HPLC指纹图谱.取大鼠100只分为正常组、模型组、各产地铁皮石斛水提取物试样组及醇提取物试样组,每组10只,正常组及模型组给予蒸馏水灌胃,各试样组分别给予相应提取物灌胃,末次给药后,除正常组外给予乙醇溶液灌胃诱导胃损伤.1h后处死大鼠,收集胃液,测定胃液分泌量及胃蛋白酶活性,检查胃损伤程度.采用偏最小二乘回归法分析谱效关系.结果 建立了8个铁皮石斛提取物的HPLC指纹图谱,并提取出30个特征峰;模型组的胃液分泌量、胃蛋白酶活性均低于正常组,而胃损伤度评分高于正常组(均P<0.05);与模型组比较,各产地铁皮石斛水提物组胃液分泌量增高而胃损伤度评分降低(均P<0.05),各产地铁皮石斛水提物及醇提物组的胃蛋白酶活性均高于模型组(均P<0.05).第4、8、15、18、21、23号色谱峰具有益胃作用.结论 铁皮石斛益胃作用的谱效相关性强,第4、8、15、18、21、23号峰所代表的化合物可能为铁皮石斛发挥益胃作用的主要有效成分.%Objective To investigate the correlation between high performance liquid chromatography (HPLC) fingerprints and stomach-nourishing effect of dendrobium offcinale.Methods The water extract and ethanolic extract were made from dendrobium offcinale from 4 regions,and then HPLC was used to construct fingerprints. A hundred rats were divided into normal group,model group,water extract and ethanolic extract of dendrobium offcinale from each region group,with 10 rats in each group. Intragastric administration of distilled water was performed in the normal group and model group.Intragastric administration of corresponding extracts was performed in each dendrobium offcinale extract group. After the last administration,intragastric administration of alcohol solution was performed for gastric injury induction in all groups except the normal group.All rats were killed after 1 hour,then the gastric fluid was collected to detect the secretory volume of gastric fluid and pepsin activity,and the gastric injury was assessed.The partial least squares regression analysis was used to analyze the spectrum-effect relationship.Results The HPLC fingerprints of extracts from 8 dendrobium offcinale were established, and 30 characteristic peaks were confirmed.In the model group,the secretory volume of gastric fluid and pepsin activity were lower and the score of gastric injury was higher compared to the normal group(all P<0.05).Compared to the model group,the secretory volume of gastric fluid was higher and the score of gastric injury was lower in each water extract of dendrobium offcinale from each region group,the pepsin activity was higher in each water extract and ethanolic extract of dendrobium offcinale from each region group(all P<0.05).No.4, 8,15,18,21 and 23 chromatographic peaks were related to stomach-nourishing effect.Conclusion The HPLC fingerprints is closely related to the stomach-nourishing effect of dendrobium offcinale.The substances No.4,8,15,18,21 and 23 chromatographic peaks representing are probably the major active ingredients of dendrobium offcinale with stomach-nourishing effect.