Objective To establish a HPLC method for the determination of peoniflorin in Danggui Yixue oral liquid.Methods The determination was performed on C18(250mm ×4.6mm,5μm).The mobile phase consisted of isopropanol-water-glacial acetic acid (4.8:95:0.2) at a flow rate of 1.5 mL・ min-1 .The detection wavelength was set at 230nm.Results The linear range of peoniflorin was 11.886μg~99.049μg(r=0.999),with average recovery at 100.68%,RSD=2.5%(n=9).Conclusion The method was proved to be simple, accurate, reproducible and specific, and it can be used for the quality control and evaluation .%目的:建立以高效液相色谱法测定当归益血口服液中芍药苷含量的方法。方法色谱柱为C18柱(250 mm ×4.6 mm,5μm),流动相为异丙醇-水-冰醋酸(4.8:95:0.2),检测波长为230 nm,流速为1.5 mL・min-1,结果芍药苷进样量在11.886μg~99.049μg范围内与峰面积积分值呈良好的线性关系(r=0.999);平均回收率为100.68%,RSD=2.5%(n=9)。结论本方法简便、准确、重现性好、专属性强,可用于该制剂的质量控制和评价。
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