胃泌素／CCK－B受体环对多种肿瘤细胞生长和凋亡的影响

摘要

目的：探讨胃泌素／CCK－B受体环对肝癌、肺癌和乳腺癌细胞生长和凋亡的影响。方法用免疫细胞化学检测肺癌A549细胞、肝癌HepG2细胞及乳腺癌MCF－7细胞系中胃泌素和CCK－B受体的表达。再用CCK－B受体拮抗剂丙谷胺（5 mmol／L）处理细胞（实验组），分别用MTT实验、流式细胞仪及分光光度法检测3种癌细胞的生长曲线、细胞凋亡及caspase－3的酶活性，对照组未用丙谷胺处理。结果肝癌HepG2细胞、肺癌A549细胞和乳腺癌MCF－7细胞中胃泌素和CCK－B受体的表达均为阳性；与对照组比较，实验组细胞的生长被显著抑制，caspase－3酶活性和凋亡细胞百分数显著升高，差异均有统计学意义（P＜0．05，P＜0．01）。结论胃泌素／CCK－B受体环参与肝癌、肺癌和乳腺癌细胞的生长和凋亡，阻断此环能抑制细胞的生长，促进细胞凋亡。%Objective To investigate the effects of gastrin/CCK-B receptor signaling on cell growth and apopto-sis in liver cancer, lung cancer and breast cancer cell lines.Methods Expression of gastrin and CCK-B receptor was detected in hepatocarcinoma HepG2 cells, lung carcinoma A549 cells and breast cancer MCF-7 cells by immunocyto-chemistry.After treatment of 5 mmol/L proglumide (experimental groups), a CCK -B receptor antagonist, the cell growth curve, apoptosis and caspase-3 activity were detected by MTT assay, flow cytometry and spectrophotometry, re-spectively.The control groups were not treated with proglumide.Results The liver cancer HepG2 cells, lung cancer A549 cells and breast cancer MCK-7 cells showed positive expression for gastrin and CCK-B receptor.After proglumide treatment, cell growth was significantly inhibited, and caspase-3 activity and percentage of apoptotic cells significantly increased in all of the experimental groups compared with the control groups (P<0.05 or P<0.01).Conclusion Gas-trin/CCK-B receptor signaling is involved in the cell growth and apoptosis in hepatocarcinoma, lung carcinoma and breast cancer and blocking of the signal pathway can inhibit cell growth and promote apoptosis.