胃泌素/胆囊收缩素受体环对胃癌细胞增殖迁移的影响

摘要

Objective To study the effects of gastrin/ CCKB receptor loop on the proliferation and migration of human gastric carcinoma cell. Methods The expression of CCKB receptor was detected in human gastric cancer cell lines SGC-7901 and AGS cells and gastric epithelial cell line GES-1 cells by immunocytochemical method. Afrer three cultured cells were treated with the final concentration of 10 nmol/L and 100 nmol/L gastrin, the cell proliferation and migration were studied by cell wound assay and MTT colorimetric assay. The cells without treatment of gastrin served as the controls. Results The expression of CCKB receptor expressed strong positively in SGC-7901 and AGS cells and weak positively in GES-1 cells. Compared with the control cells, the abilities of the proliferation and migration were significantly increased in gastric cancer AGS and SGC-7901 cells, but had no significant change in GES-1 cells after treatment of gastrin with the final concentration of 10 nmol/L and 100 nmol/L. The relative proliferation rate was 100% , 191. 13% and 212.65% in SGC-7901 cells and 100% , 189. 27% and 209. 19% in AGS cells in the control, 10 nmol/L and 100 nmol/L gastrin groups, respectively. The difference was statistically significant ( all P <0. 01) . But the relative proliferation rate in GES-1 cells was 100% , 113.01% and 116. 12% in the control, 10 nmol/L and 100 nmol/L gastrin groups respectively, which had no statistically significance (all P > 0.05). Conclusion The expression level of CCKB receptor have an important role in the proliferation and migration of gastric cancer cells promoted by gastrin/CCKB receptor loop.%目的 探索胃泌素/胆囊收缩素受体环对胃癌细胞增殖迁移的影响.方法 用免疫细胞化学方法检测人胃癌细胞SGC-7901、AGS和胃黏膜上皮细胞GES-1细胞中胆囊收缩素-B(CCKB)受体的表达.分别培养这3种细胞,用不同终浓度胃泌素(10、100nmol/L)处理细胞,以未加胃泌素的细胞为对照,用细胞划痕实验和四甲基偶氮唑盐比色法检测细胞的增殖迁移能力.结果 胃癌细胞SGC-7901、AGS中CCKB受体表达强阳性,而GES-1细胞中CCKB受体表达弱阳性；不同浓度胃泌素处理细胞后,SGC-7901、AGS细胞的增殖迁移能力明显增强,而GES-1细胞无明显改变；对照组、10 nmol/L胃泌素处理组及100 nmol/L胃泌素处理组中SGC-7901细胞的相对增殖率分别为100％、191.13％、212.65％,AGS细胞的相对增殖率分别为100％、189.27％、209.19％,各组间差异均有统计学意义(P均＜0.01)；而GES-1细胞的相对增殖率分别为100％、113.01％、116.12％,各组间差异无统计学意义(P均＞0.05).结论 CCKB受体表达水平的高低对胃泌素/CCKB受体环促进胃癌细胞的增殖和迁移起重要作用.