肥胖复合牙周炎大鼠骨骼肌中瘦素受体表达的组织学研究

摘要

Objective To invesitgate the protein expression for leptin receptor in skeletal muscle in periodontitis and obese rat models,with the aim of exploring the effect of experimental periodontitis on adipokines receptors in insulintargeting tissues and underlying mechanisms of skeletal muscle insulin resistance.Methods Forty natal SD male rats were randomly divided into four groups,respectively:obese rats with periodontitis (OB+ CP group),obese rats without periodontitis (OB group),normal rats with periodontitis (CP group) and normal rats without periodontitis (C group).Obese rat model was established by subcutaneous injection of sodium glutamate.Experimental periodontitis was induced by ligation and inoculation of periodontal pathogens.Immunohistochemical analysis of leptin receptor expression in skeletal muscle of four groups was employed.Results In the skeletal muscle,the protein for leptin receptor in the C group and CP group were popular and strong positive,and there was no statistically significant difference between the groups (P ＞ 0.05).The protein expressions in the OB + CP group and OB group were decreased.Fewer protein expression was detected in the OB + CP group when compared with OB group (P ＜ 0.05).A synergistic interaction between obesity and periodontitis was evident to the expression for the leptin receptor (P ＜ 0.05).Conclusion Local infection induced by experimental periodontitis can barely down-regulate the protein expression for leptin receptor in the skeletal muscle.However,periodontitis can significantly down-regulate the protein expressions with the presence of obesity.%目的 探讨牙周炎对胰岛素靶组织中脂肪细胞因子受体表达的调控及其在骨骼肌胰岛素抵抗中的作用.方法 将40只新出生的雄性SD大鼠随机分为4组:肥胖合并牙周炎组(OB+ CP),肥胖组(OB),牙周炎组(CP)和正常对照组(C).通过皮下注射谷氨酸钠建立肥胖模型;丝线结扎联合局部接种牙周致病菌建立牙周炎模型.采用免疫组化染色法检测4组大鼠骨骼肌组织中瘦素受体的表达.结果 瘦素受体在C组和CP组的骨骼肌中广泛表达,组间差异无统计学意义(P＞0.05);而在OB组和OB+ CP组骨骼肌组织中表达量减少,OB+ CP组中瘦素受体的表达量低于OB组,差异有统计学意义(P＜0.05).肥胖和牙周炎对骨骼肌中瘦素受体的表达存在交互效应(P＜0.05).结论 牙周炎的局部感染不能直接影响大鼠骨骼肌组织中瘦素受体的表达,但牙周炎可使肥胖机体骨骼肌中瘦素受体的表达下调.