异叶三宝木中倍半萜类化学成分研究

摘要

综合运用硅胶柱色谱、ODS反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱及制备型高效液相等现在色谱分离技术对大戟科三宝木属植物异叶三宝木Trigonostemon heterophyllus枝叶中的化学成分进行系统的分离和纯化.根据化合物的理化性质和波谱数据,并通过与文献对照,鉴定了从异叶三宝木枝叶85 %乙醇提取物的石油醚萃取部位中分离得到的8个倍半萜类化合物,分别鉴定为:9-hydroxy-4,7-megastigmadien-3-one (1)、9,10-dihydroxy-4,7-megastigmadien-3-one (2)、blumenol A (3)、4, 5-dihydroblumenol A (4)、dehydrovomifoliol (5)、3-hydroxy-β-ionone (6)、sarmentol F (7) 和5,12-epoxy-9-hydroxy-7-megastigmen-3-one (8).所有化合物均为首次从三宝木属植物中分离得到.%The chemical consituents from stems and leaves of Trigonostemon heterophyllus were separated and purified by column chromatographies with silica gel, ODS, Sephadex LH-20 and Pre-HPLC. The structures of the isolated compounds were identified on the basis of physicochemical properties and spectroscopic analysis, as well as comparisons with the data in literature. Eight compounds were isolated and elucidated as 9-hydroxy-4,7-megastigmadien-3-one (1), 9,10-dihydroxy-4,7-megastigmadien-3-one (2), blumenol A (3), 4, 5-dihydroblumenol A (4), dehydrovomifoliol (5), 3-hydroxy-β-ionone (6), sarmentol F (7) and 5,12-epoxy-9-hydroxy-7-megastigmen-3-one(8).All compounds were isolated from the genus Trigonostemon for the first time.