长枝木霉对白三叶种子发芽及幼苗抗逆性研究

摘要

The effect of Trichoderma longibrachiatum on seeds growth and resistance of clover was studied by measuring the germination rates,root length and resistance of clover treated with different spores concentra-tions of T.longibrachiatum.The results showed that the vigor of clover seeds was increased after treated with the concentration of 5.0 × 10 5 to 1.0 × 10 7 spores/mL of T .longibrachiatum.The germination rates,root length,shoot length were 93%,1.93 cm and 1.33 cm after treated with the concentration of 1.0 × 10 6 spores/mL,and which were increased by 1 6.25%,53.1% and 27.8% compared to control(80%,1.26 cm,1.04 cm),respectively.The spore suspension of T .longibrachiatum could enhance the ability of seeds resistance to plant disease and unfavorable environment.The activity of phenylalanine(PAL)and peroxidase(POD)were in-creased after treated with the spore suspension,but the activity of methane dicarboxylic aldehyde(MDA)was decreased.The maximum activity of PAL and POD were 2.157 U/g(FW)·min and 29.875 U/g(FW)while the spore suspension concentrations were 5.0×10 5 and 1.0×10 6 spores/mL,respectively.The minimum activity of MDA was 1.012μmol/g(FW)treated with 5.0×10 6 spores/mL.%在室内条件下,用不同浓度的长枝木霉孢子悬浮液对白三叶种子进行处理,研究了其种子发芽率、根芽长及抗逆性酶活变化,以及长枝木霉对白三叶种子的促生作用及抗逆性的影响。结果表明,长枝木霉孢子悬浮液浓度在5.0×105个/mL~1.0×107个/mL 时能促进白三叶种子活性,在孢子浓度为1.0×106个/mL 时,发芽率为93%,与对照80%相比提高了16.25%；胚根长1.93 cm,与对照1.26 cm 相比增长了53.1%；胚芽长1.33 cm,与对照1.04 cm 相比增长了27.8%。抗逆性结果表明,长枝木霉孢子悬浮液能够提高白三叶种子抗病害及不良环境的能力,降低 MDA 含量,提高 PAL、POD 的活性。其中,孢子悬浮液浓度为5.0×105个/mL 时,PAL 活性最高,为2.157 U/(g·min)FW；孢子浓度为1.0×106个/mL 时 POD 的活性最高,为29.875 U/g FW；孢子悬浮液浓度5.0×105个/mL 时,MDA含量最低,为1.012μmol/g FW。