NO对低温胁迫下草地早熟禾抗氧化r系统的调控

摘要

Effects of endogenous nitric oxide on antioxidant system of kentucky bluegrass (Poa Pratensis L) under low temperature stress in artificial indoor simulation was studied in this paper,and kentucky bluegrass was collected from Dingxi city,Gansu Province.The results showed that sodium nitroprusside (SNP)treatment decreased the content of malondialdehyde (MDA),H 2 O2 and OH,and O2 - emission rate under,while promoted the activity of superoxide dismutase (SOD),peroxidase (POD),ascorbate oxide enzyme (APX)and glutathione reductase (GR).The free radical and protective enzyme activities were unchangeable under SNP treatment.Bo-vine hemoglobin (used as scavenging agent),tungstate (used as nitrate reductase activity inhibitor)and N-nitro-L-arginine methyl ester (used as nitric oxide synthase inhibitor)reversed of nitric oxide donor SNP effects.Ni-tric oxide promoted the antioxidant enzyme activities of kentucky bluegrass under low temperature stress,and decreased the lipid peroxidation and maintain membrane stability and increased the adaptability of kentucky bluegrass under low temperature stress.%以草地早熟禾为材料,人工室内模拟低温逆境环境,研究了NO对低温胁迫下草地早熟禾抗氧化系统的影响.结果表明,SNP处理降低了低温胁迫下草地早熟禾MDA、H 2 O2、OH含量及O2-产生速率,提高了SOD、POD、APX和GR活性,NO供体SNP的类似物亚铁氰化钠对低温胁迫下草地早熟禾自由基和保护酶活性无明显影响.施用NO清除剂Hb、硝酸还原酶抑制剂钨酸钠和一氧化氮合酶抑制剂N-硝基-L-精氨酸甲脂具有逆转NO供体SNP的效应.说明NO可以通过促进低温胁迫下草地早熟禾抗氧化酶活性的提高,来降低膜脂过氧化水平,维持膜稳定性,增强草地早熟禾对低温胁迫的适应性.