HPLC法同时测定复方金思维中3种有效成分的含量

摘要

目的：建立复方金思维中松果菊苷、毛蕊花糖苷、3,6＇-二芥子酰基蔗糖的含量测定方法。方法采用高效液相色谱法对制剂中的松果菊苷、毛蕊花糖苷、3,6＇-二芥子酰基蔗糖进行定量测定。结果松果菊苷、毛蕊花糖苷、3,6＇-二芥子酰基蔗糖分别在2.5~50.0μg/mL、1.3~25.9μg/mL、2.0~40.7μg/mL范围内与峰面积线性关系良好,3种成分样品回收率分别为101.37％、99.86％和98.44％,RSD均在3.00％以内。结论所建立的方法简便,重复性良好,可用于金思维复方中松果菊苷、毛蕊花糖苷、3,6＇-二芥子酰基蔗糖的含量测定。%Objective To establish a method for determination of 3 effective components in Compound JSW prescription. Methods The contents of Echinacoside, Acteoside, 3, 6'-Disinapoyl sucrose in Compound JSW prescription were determined by HPLC. Results Echinacoside, acteoside, 3,6'-Disinapoyl sucrose showed good linear correlation within 2. 5~50. 0 μg/ml, 1. 3~25. 9 μg·ml-1 and 2. 0~40. 7μg/ml(r≥0. 9999),respectively. The average recoveries were within 101. 37%, 99. 86%and 98. 44%, respectively. And RSD were less than 3. 00%. Conclusion The quantitative method for determining the components of Compound JSW prescription is simple, feasible and repeatable.