人参皂苷Rd治疗大鼠溃疡性结肠炎给药途径的研究

摘要

Objective:The aim of the study was to investigate the therapeutic effect of administration route of ginsenoside Rd in the treatment of ulcerative colitis (UC). Methods:Colitis in rats was induced by colonic administration with 2,4,6-trinitrobenzene sulfonic acid (TNBS).Wistar rats were randomly divided into three groups:TNBS control group, ginsenoside Rd intragastric group (ig at 30mg/kg ginsenoside Rd) and intramuscular injection group(im at 30mg/kg ginsenoside Rd). Rats were administrated by ginsenoside Rd(30mg/kg) per day for 7days with different administration route. Macroscopic and microscopic damage scores and changes in weight/length ratio(mg/mm) of colon segments were analyzed. Results:1.Compared with the TNBS control group, Rd ig group and im group facilitated recovery of pathologic changes in the colon and decreased colonic weight/length ratio (P<0.01 or P<0.05). 2.Rd also notably reduced the colonic macroscopic and microscopic damage scores (P<0.01). Conclusion:Rd exerts a beneficial effect in the TNBS-induced colitis in rats and compared with intramuscular injection, the administration of Rd by ig has a better effect on ulcerative colitis in rats .%目的：：研究人参皂苷Rd（Rd）治疗大鼠溃疡性结肠炎（UC）的最佳给药途径。方法：用炎症诱导剂三硝基苯磺酸（TNBS）溶液诱导法建立大鼠溃疡性结肠炎模型。将Wistar雄性大鼠随机分为模型对照组、Rd灌胃给药组、Rd肌肉注射组。Rd给药剂量为30 mg/kg，每天给药1次，连续给药7天。结果：①与模型对照组比，Rd灌胃给药组及Rd肌肉注射组大鼠结肠湿重指数均明显减轻，差异具有统计学意义（P<0.01或P<0.05），其中灌胃给药组大鼠结肠湿重指数最低。②与模型对照组比，Rd灌胃给药组及Rd肌肉注射组能够明显降低UC大鼠结肠大体观察评分及病理组织学评分，减轻大鼠结肠的病理学损伤，差异具有统计学意义（P<0.01）。结论：人参皂苷Rd对大鼠溃疡性结肠炎有明显的治疗作用，且灌胃给药作用更强。